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FirstDraft™ cDNA Libraries

  • Unique cloning format preserves the rarest sequences
  • Ideal for identifying alternative splice isoforms
  • High representation of full-length clones for functional analysis
  • Limited edition comprised of authentic primary clones
  • Provided as glycerol stocks and super-coiled DNA for direct use
  • Cloning method prevents genomic and chimeric artifacts
The FirstDraft™ cDNA libraries provide a valuable source for the discovery of new genes, and as evidence for predicted proteins and alternative splice isoforms. To provide a discovery system with the most complete representation of expressed genes, each FirstDraft™ product is comprised of two main libraries derived from different cDNA sizing procedures (Low Sizing >800 bp, and High Sizing >2000 bp). The libraries are offered as primary clones that have never been amplified, preserving the representation of the rarest and most fragile mRNA sequences.
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The FirstDraft™ product is provided in a format containing 48 sub-pools and 2 master pools-where purified DNA and a glycerol stock is supplied for every pool (Unique Library Format). This unique format increases the probability of finding rare and novel genes, and is particularly useful for the discovery of new splice isoforms and gene-family members. The DNA is ready to use and suitable for PCR cloning, in vitro RNA transcription and transfer into other systems for protein interaction studies.

Collection of FirstDraft™ cDNA Libraries: A Limited Edition
The FirstDraft™ cDNA Libraries are a unique collection of high-quality plasmid cDNA libraries. The collection includes several libraries of Human cancer cell lines derived from breast, colon, lung, ovarian, prostate and renal cancer, as well as, leukemia and melanoma. A total of 28 different cancer cell lines are represented from the NCI60 list-the set of 60 human cancer cell lines selected by the National Cancer Institute (NCI) for pharmacological studies. An extraordinary Human Brain Library derived from 12 distinct regions is also offered, as well as, a selection of rat libraries. These libraries are also available as Master Pools Only (MPO), which are designated by the corresponding MPO catalog number. Custom FirstDraft™ cDNA Libraries are also available, to inquire please e-mail us at custom@genofi.com.

FirstDraft™ cDNA Libraries Source material Library Analysis Catalog # Price (US)
Human Leukemia Cell Lines 6 cell lines: 29SR, CCRF-CEM, HL-60, K-562, Molt-4, RPMI-8226 See Details FDH-701
MPO-701
$5,350
$2,950
Human Colon Cancer Cell lines 5 cell lines: Colo-205, DLD-1, HCT-15, HCT-116, SW-620 See Details FDH-702
MPO-702
$5,350
$2,950
Human Lung Cancer Cell Line 4 cell lines: A549, DMS114, NCI-H460, SHP-77 See Details FDH-703
MPO-703
$5,350
$2,950
Human Breast Cancer Cell lines 7 cell lines: BT-549, Hs 578T, MCF7, MDA-MB-231, MDA-MB-435S, MDA-MD-468, T-47D See Details FDH-704
MPO-704
$5,350
$2,950
Human Other Cancer Cell Lines 6 cell lines: melanoma RPMI-7951, ovarian OVCAR-3, prostate PC-3 & DU 145, renal 786-O & ACHN See Details FDH-705
MPO-705
$5,350
$2,950
Human Brain 12 Areas Whole Brain, Amygdala, Caudate Nucleus, Hippocampus, Insula, Medulla Oblongata, Nucleus Accumens, Paracentral Gyrus, Pons, Postcentral Gyrus, Putamen, Subthalamic Nucleus See Details FDH-706
MPO-706
$5,350
$2,950
Rat Brain Adult
(Brown Norway)
Total brain (2 male adults) See Details FDR-601
MPO-601
$5,350
$2,950
Rat Total Embryo
(Brown Norway)
Total embryos (16-18 dpc) See Details FDR-602
MPO-602
$5,350
$2,950
Rat Adult Universal
(Brown Norway)
Equal ratio of male/female: 80% total animal, 10% brain, 5% placenta and 5% testes See Details FDR-603
MPO-603
$5,350
$2,950